Fig. 5

Canonical Smad signalling promotes neurite growth in SCG neurons. a Smad-dependent transcriptional activity in P0 SCG neurons 24 h after transfection with the Smad-GFP reporter plus either the caBMPR-IB plasmid or pcDNA3.1 control plasmid and either Smad decoy oligonucleotides or scrambled control decoy oligonucleotides. b Length, c branch number and d Sholl analysis of the neurite arbors of P0 SCG neurons 24 h after transfection with the indicated plasmids and oligonucleotides. For clarity, the Sholl plot includes only one control plasmid condition. e Photomicrographs of representative P0 SCG neurons 24 h after transfection with the plasmids indicated. Mean ± SEM of data from at least 150 neurons in each condition from 3 independent experiments are shown (*** P < 0.001, statistical comparison with control, one-way ANOVA with Fisher’s post hoc)