Evx1 and Evx2 specify excitatory neurotransmitter fates and suppress inhibitory fates through a Pax2-independent mechanism

Table 1 Number of cells expressing particular genes and proteins in WT and mutant embryos

Marker	Stage	WT	evx1 mutants	P^a	evx2 mutants	P^b	Double mutants	P^c	P^d	P^e
evx1	24 h	33.0 + /-2.0	31.0 + /-3.3	0.32	31.3 + /-2.5	0.56	17.4 + /-2.6	<0.01	<0.01	<0.01
evx2	24 h	28.6 + /-1.6	22.4 + /-50	0.02	13.5 + /-3.5	<0.01	0.00	<0.01	<0.01	<0.01
Evx2	30 h	33.6 + /-5.2	23.0 + /-4.6	0.01	N.D		N.D
eng1b	24 h	43.3 + /-2.5	43.6 + /-2.0	0.80	43.5 + /-4.0	0.94	37.2 + /-6.0	0.08	0.07	0.10
eng1b	30 h	63.0 + /-3.0	62.0 + /-2.5	0.63	63.0 + /-2.5	0.92	65.2 + /-2.0	0.15	0.06	0.15
slc17a6 (vlgut)	24 h	89.0 + /-10.0	71.3 + /-4.3	<0.01	74.6 + /-8.0	0.01	60.8 + /-10	<0.01	0.08	0.04
slc32a1 (viaat)	24 h	157.4 + /-8.0	157.3 + /-10.0	0.75	158.2 + /-4.0	0.86	178.6 + /-7.0	<0.01	<0.01	<0.01
gads (GABAergic)	24 h	50.0 + /-3.0	49.0 + /-4.0	0.59	51.6 + /-2.6	0.31	50.6 + /-2.2	0.66	0.38	0.5
slc6a5 (glyt2a/glyt2b)	24 h	81.0 + /-3.3	93.0 + /-9.0	<0.01	87.6 + /-4.5	0.02	109 + /-10.0	<0.01	0.02	<0.01
pax2a	24 h	50.6 + /-2.6	50.4 + /-2.0	0.86	49.5 + /-30	0.55	50.8 + /-2.5	0.91	0.75	0.49
Pax2	24 h	40.3 + /-3.8	39.8 + /-5.7	0.83	36.9 + /-6.9	0.23	38.8 + /-5.3	0.41	0.68	0.52
skor2	30 h	24.3 + /-3.0	15.0 + /-5.0	<0.01	19.8 + /-4.0	0.07	0.0	<0.01	<0.01	<0.01
skor2 Total cell counts	30 h	46.7 + /-3.7	38.7 + /-3.3	<0.01	42.4 + /-1.7	0.04	22.6 + /-1.7	<0.01	<0.01	<0.01

Numbers of cells expressing particular markers (first column on left) in spinal cord region adjacent to somites 6-10 and P values of comparisons between embryos with different genotypes. Values are shown as the mean from at least 5 different embryos +/- standard deviation, except for pax2a and eng1b where 4 embryos were counted. P values are from student’s t-tests. Statistically significant (P < 0.05) values are indicated in bold. P^a compares evx1 single mutants with WT embryos, P^b compares evx2 single mutants with WT embryos, P^c, P^d and P^e are for comparisons between double mutant embryos and WT (P^c), evx1 single mutant (P^d) and evx2 single mutant (P^e) embryos respectively. Mean cell count values are provided to one decimal place and P values to two decimal places. For skor2, two sets of values are provided: just the ventral domain of expression and both the ventral and dorsal domains of expression (total cell counts)

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