Fig. 5

Expression patterns of trigeminal ganglion-specific markers. A) Wholemount in situ hybridization of ISL1at HH21 to provide anatomical localisation of all cranial sensory ganglia. A’-A”’) Transverse sections stained with ISL1at HH21 to provide comparative sections for other markers at the level of Tmm ganglion (A’); VA ganglion (A”); and P ganglion (A”’). B-F) Wholemount in situ hybridization of trigeminal ganglion specific markers at HH21. B’-F”’) Transverse sections at the level of the Tmm ganglion (B’-F’), VA ganglion (B”-F”) and P ganglion (B”’-F”’) stained with the named marker at HH21, showing Tmm-specific expression. B-B”’) DRG11; C-C”’) PRDM12; D-D”’) GAD2; E-E”’) CHRNA3; F-F”’) CHRNB4. Levels of staining in the trigeminal ganglion vary, but are stronger when compared with other ganglia. Staining can be seen in the NT although specific localisation and level varies. The dark staining in the eye and OV in D-F was not observed on sections and likely represents background. Abbreviations: G: geniculate ganglion; N: nodose ganglion; NT: neural tube; OV: otic vesicle; P: petrosal ganglion; r: rhombomere; Top: trigeminal ophthalmic lobe; Tmm: trigeminal maxillomandibular lobe; VA: vestibulo-acoustic ganglion. Scalebars: A-F: 500 μm; A’-F”’: 150 μm