Fig. 4

Topographic mapping in wild type and ephrin-A2/A5 double knockout mice. a Schematic diagram showing the expected outcome of ventral dye placements in wild type mice, with labeled calyces seen in the lateral portion of the contralateral MNTB. b Labeled calyces in MNTBc after a ventral dye placement in VCN in a wild type animal. The normalized location of a calyx along the mediolateral axis is denoted with an asterisk; normalized positions were averaged to obtain values for each animal. As expected, the calyces are clustered in the lateral portion of MNTBc. c No calyces are seen in the MNTBi. d Section through the VCN of this animal shows the dye placement in the ventral region, with the center indicated (asterisk). e Schematic diagram depicting dye labeling in an ephrin-A2/A5 double knockout mouse. Dorsal dye placement into the high frequency region of VCN results in labeled terminations in the medial regions of both MNTBc and MNTBi. f Labeled calyces in MNTBc after a dorsal dye placement in VCN in an ephrin-A2/A5 double knockout mouse. Calyces appear clustered in the medial region. g Ipsilateral calyces are found in medial MNTB regions after dorsal dye placement in ephrin-A2/A5 double knockout mice. h Section through the VCN of this animal shows the dye placement in the dorsal region, with the center indicated (asterisk). Scale bar in G indicates 100 μm for B, C, F, and G. Scale bar in H indicates 100 μm for D and H