Table 2 Primers for mutagenesis
From: Developmental attenuation of N-methyl-D-aspartate receptor subunit expression by microRNAs
Mutated sequence | Primer (5′…3′) |
|---|---|
FLuc-Grin2a-mut 3′UTR-F | gggaggtactttttgttggctcttttaagcatggctccatgccataat |
FLuc-Grin2a-mut 3′UTR-R | attatggcatggagccatgcttaaaagagccaacaaaaagtacctccc |
FLuc-Grin2b-mut1 3′UTR-F | tggctcccattcgctttttcttcttcttttaatgcttctatgggatcctggag |
FLuc-Grin2b-mut1 3′UTR-R | ctccaggatcccatagaagcattaaaagaagaagaaaaagcgaatgggagcca |
FLuc-Grin2b-mut2 3′UTR-F | aaccctcgtggccagcaccatgcttcctccctcgc |
FLuc-Grin2b-mut2 3′UTR-R | gcgagggaggaagcatggtgctggccacgagggtt |
FLuc-Rest-mut 3′UTR PartA-F | ctttggtgcttttctggcttaagatgttaagcatggttcttgtttttgtttctttaacc |
FLuc-Rest-mut 3′UTR PartA-R | ggttaaagaaacaaaaacaagaaccatgcttaacatcttaagccagaaaagcaccaaag |
FLuc-Rest-mut1 3′UTR PartB-F | taactaagtacagtccggattttaagtatcacattgcttcagggatctccacaaac |
FLuc-Rest-mut1 3′UTR PartB-R | gtttgtggagatccctgaagcaatgtgatacttaaaatccggactgtacttagtta |
FLuc-Rest-mut2 3′UTR PartB-F | gttactttcctgaggcaaaccaaaggtatatgcttcaaggttctgctgcc |
FLuc-Rest-mut2 3′UTR PartB-R | ggcagcagaaccttgaagcatatacctttggtttgcctcaggaaagtaac |