Fig. 4
From: Developmental attenuation of N-methyl-D-aspartate receptor subunit expression by microRNAs
The role of REST in miRNA regulation of NMDAR subunit expression. a. Putative miR-19a and miR-539 binding sequences in the 3′UTR of REST. b. qPCR analysis of REST mRNA expression. Its expression markedly decreased from P1 to P11 followed by a plateau into the adult (P60). REST negatively correlates with miR-539 (The Pearson’s correlation coefficient is r = -0.5747 for REST/miR-539). c. PartA of the REST 3′ UTR contains a MRE for miR-19a and PartB contains two MREs for miR-539. Only the miR-539 mimic effectively targeted the 3′UTR of REST containing the MREs for miR-539. A weak increase was observed on the Luc-REST-mut 3′UTR with the miR-539 mimic (n = 3) (mut.: 3′UTR mutated)