Fig. 3
From: Developmental attenuation of N-methyl-D-aspartate receptor subunit expression by microRNAs
a, b . Relative mRNA levels obtained for Grin2a and Grin2b in rat hippocampal cultures. RNA was extracted between DIV3 (Day In Vitro 3) and DIV20. The values represented are normalized to GAPDH by SYBR qRT-PCR. The expression of their corresponding miRNAs (miR-19a and miR-539) are also represented and normalized to U6. c. Protein expression obtained for GluN2A, GluN2B and the loading control β-actin by Western-Blot. d. Expression of the GluN2A and GluN2B proteins after miRNA inhibitor treatment by Western blot. At DIV8, the rat hippocampal neurons were treated with 25 nM, 50 nM or 75 nM of miR-19a, miR-539 inhibitors, with a control (Ctrl) or untreated (Un.).Data in histograms were quantified with Image J software (n = 3). The values for the protein levels are normalized to β-actin