Fig. 2

a. Validation of the Grin2a mRNA 3′UTR as a miR-19a target: relative Grin2a mRNA 3′UTR luciferase levels with 50 nM or 100 nM of miR-19a mimic measured 24 hrs after transfection (Mut., mutant UTR with a 3 bp mutation of the miR-19a target site; Ctrl, values obtained with the mirVana miRNA mimic Control) and relative Grin2a mRNA 3′UTR luciferase levels 24 h after transfection with an anti-miR-19a (concentrations of 50 nM and 100 nM) (Ctrl, transfection with a LNA Inhibitor Control). b. Validation of the Grin2b mRNA 3′UTR as a miR-539 target: relative Grin2b mRNA 3′UTR luciferase levels with 100 nM or 150 nM of miR-539 mimic 24 h after transfection (the concentrations used are higher than those for miR-19a because the Grin2b mRNA 3′UTR possesses two miR-539 target sites) (Mut., mutant UTR with a 3 bp mutation of the miR-539 target site; Ctrl, values obtained with the mirVana miRNA mimic Control) and relative Grin2b mRNA 3′UTR luciferase levels 24 h after transfection with an anti-miR-539 (concentrations of 100 nM and 150 nM) (Ctrl, transfection with a LNA Inhibitor Control). c. miR-19a and miR-539 binding sequences in the 3′UTR of Grin2a and Grin2b respectively, and the corresponding seed matches