Fig. 1
Melanopsin positive cell density decreases with age. a melanopsin and SMI-32 co-labeling in P8 and P30 retinas. Images emphasize either cell somata or cell dendrites. Arrowheads highlight co-labeled cells while line arrows highlight M1 cells. Scale bar = 50 μm. b Average densities of melanopsin-only positive cells (bottom bars) and melanopsin/SMI-32 co-labeled cells (top bars) in P8, P15 and P30 (n = 16 samples for each age). No significant difference in melanopsin+/SMI-32−. For melanopsin+/SMI-32+ cells ANOVA, Bonferroni post hoc *1: p = 0.0003, *2: p = 7.8×10−8, *3: p = 0.056. c Relative pixel intensities for M1, M2 and M4 somata and dendrites at P8, P15 and P30. Data is ratio of a cell structure average pixel intensity to the average pixel intensity of the brightest cell soma measured in the same image. For each subtype/age category n = 24. *1: p = 9.6×10−9, *2: p = 1.0×10−7, *3: p = 0.058, *4: p = 0.050, *5: p = 0.009, *6: p = 0.002, *7: p = 6.9×10−9, *8: p = 8.4×10−9, K-W, followed M-W and B-c