Dynamic regulation of mRNA decay during neural development

Table 1 Whole embryo mRNA decay analysis: functional annotation of high-stability and low-stability mRNAs

Whole embryo measurements: high-stability mRNAs
GO term	Definition	P value	FDR	Count	Fold enrichment
0022626	Cytosolic ribosome	2 × 10⁻⁵⁷	5 × 10⁻⁵⁶	67	10.3
	RpL4, RpL23, RpS16, RpS24
0000022	Microtubule cytoskeleton	8 × 10⁻¹⁷	3 × 10⁻¹⁴	57	3.5
	Tub84b, Tub56D, ssp4, robl
0022900	Electron transport chain	1 × 10⁻¹⁴	2 × 10⁻¹²	29	5.9
	mt:Cyt-b, mt:CoI, mt:CoII, mt:ND1
0031497	Chromatin assembly	2 × 10⁻⁹	2 × 10⁻⁷	15	7.8
	His1, His2A, His2Av, His2B, His3
0045941	Positive regulation of transcription	4 × 10⁻³	.14	15	2.4
	DSIF, HP1, Trl, ash2, lid, arm, btd
0048024	Regulation of mRNA splicing	7 × 10⁻³	.19	10	2.9
	Hrb27c, Hrb87f, B52, SC35, snf
Whole embryo measurements: low-stability mRNAs
GO term	Definition	P value	FDR	Count	Fold enrichment
0004672	Protein kinase activity	2 × 10⁻⁹	1 × 10⁻⁶	41	2.6
	S6kII, BubR1, mkk4, dTOR, wts, htl
0048812	Neuron projection morphogenesis	2 × 10⁻⁸	2 × 10⁻⁶	40	2.7
	Con, trio, Ephrin, Eph, otk, drl, rl
0006351	Transcription	1 × 10⁻⁵	4 × 10⁻⁴	47	1.8
	svp, dpn, Myc, grn, kn, Trx, eve
0019226	Transmission of nerve impulse	3 × 10⁻⁵	9 × 10⁻⁴	26	2.6
	CASK, cha, synaptogyrin, cpx, shi
0014706	Striated muscle tissue development	3 × 10⁻⁵	1 × 10⁻³	11	5.3
	Hem, Mef2, Mlp84B, flw, mbc
0051674	Localization of cell	5 × 10⁻⁴	.01	23	2.3
	cbl, APC, Dl, Mhc, baz, dome, cv-c

Gene ontology categories enriched among the 500 most stable and 500 least stable transcripts were identified using DAVID. Representative genes are listed for each category. FDR is the Benjamini-Hochberg false discovery rate.

ISSN: 1749-8104