Figure 1
From: Subrepellent doses of Slit1 promote Netrin-1 chemotactic responses in subsets of axons
Principle of the microfluidic device. Top (A) and side (B) view of the microfluidic device. The explant is cultured in a microwell, and the neuronal culture coverslip is placed in contact with microchannels interfaced with a porous membrane for the experiment. A gradient forms over the microwell. (C) The concentration profile at the coverslip surface, perpendicular to the channel axis, in the center of a microwell, measured by TIRFM (blue dots) and obtained by simulation using a diffusion model (red squares).