Figure 4
From: Multiple conserved regulatory domains promote Fezf2 expression in the developing cerebral cortex
Enhancer 434 is active in cortical progenitor cells. (A-C) Immunohistochemistry demonstrated LacZ expression in progenitor cells at E11.5 that co-expressed SOX2 (A), PAX6 (B) and PHH3 (C). LacZ was also expressed in a few postmitotic neurons in the preplate marked by Tuj1 (D). X-Gal staining of whole mount P0 brains (E) and sections (F) demonstrated reporter activity in the VZ. (G, H) Immunohistochemistry using ß-Gal antibody combined with antibodies for RGC markers BLBP (G) and PAX6 (H). (I, K, L) In adult animals, the majority of enhancer 434-LacZ+ cells were in GFAP+ astrocytes throughout the forebrain. A subset of non-astrocyte like cells expressed SATB2 (J, J’). Arrows represent co-expression of LacZ and the indicated markers. Hip, hippocampus; WM, white matter. Scale bars: (D) 50 μm, (E) 250 mm, (F) 500 μm, (G) 25 μm, (H) 10 μm, (I) 500 μm, (J) 100 μm, (J’) 50 μm, (K) 25 μm.