Figure 7

Myosin ii and F-actin dynamics motor activity is required for basal motility of cilia. Cilia were imaged for 1 hour in control, jasplakinolide-treated, and blebbistatin-treated non-migrating CGNs. The average velocity was measured before and at various time points after addition of cytoskeletal drugs. (A) In control neurons, mean cilia velocity was similar at all time points (mean velocity at each time point ranged from 0.028 to 0.024 (±0.010) μm/sec, n = 104 to 144 cilia per time point). Below, motility of the cilia before treatment and at 60 minutes is supported by representative imaging sequences showing the temporal positions of the cilia (position of the organelle is shown at every 75 seconds in a different color as indicated by the key). Cilia appear to be moving similarly in both images. (B) Cilia velocity rapidly diminished after treatment with 50 μM blebbistatin; the cilia were motile before treatment (mean velocity, 0.028 ± 0.011 μm/sec, n = 133 cilia) and almost stationary after 60 minutes of treatment (mean velocity, 0.008 ± 0.003 μm/sec, n = 124 cilia). (C) Cilia velocity also rapidly diminished after treatment with 5 μM jasplakinolide; the cilia were motile before treatment (mean velocity, 0.030 ± 0.015 μm/sec, n = 122 cilia), but mean velocity was reduced to 0.012 ± 0.005 μm/sec (n = 138 cilia) after 60 minutes of treatment.