Table 1 RGC burst and wave properties recorded across development in control and Ferret VAChT-Sap-treated retinae

Total Neurons

257

316

247

418

463

355

334

245

175

184

Total Neurons Analyzed for Bursts

255

316

244

415

457

336

328

241

175

179

Bursts Per Neuron

10.04 ± 1.21

12.34 ± 1.42

11.47 ± 3.28

14.98 ± 4.81

10.37 ± 2.16

12.36 ± 1.22

8.36 ± 0.76

16.88 ± 4.33*

7.66 ± 1.40

16.23 ± 4.27

Burst Frequency (bursts/min)

0.67 ± 0.08

0.82 ± 0.09

0.76 ± 0.22

1.00 ± .32

0.69 ± 0.14

0.82 ± 0.08

0.55 ± 0.05

1.12 ± 0.29*

0.51 ± 0.09

1.08 ± 0.28

% Spikes in Bursts

99.14 ± 0.10

98.13 ± 0.55

97.67 ± 0.79

96.49 ± 1.82

98.18 ± 0.61

95.28 ± 1.60

97.96 ± 0.65

94.69 ± 1.19*

96.04 ± 1.58

91.50 ± 2.54

Maximum Burst Spike Rate

459.76 ± 63.76

473.06 ± 162.98

755.15 ± 187.18

467.56 ± 155.52

528.04 ± 85.41

528.48 ± 161.02

510.52 ± 62.51

438.55 ± 199.77

643.55 ± 115.61

623.60 ± 292.19

% of Total Burst Time >10 Hz

58.97 ± 4.10

57.51 ± 4.54

57.03 ± 4.44

53.48 ± 3.53

61.42 ± 3.60

42.82 ± 3.54***

67.42 ± 2.8

40.14 ± 4.43***

64.59 ± 0.53

33.53 ± 4.52***

% Bursts in Waves

87.77 ± 2.76

80.62 ± 6.6

83.95 ± 2.48

77.20 ± 4.69

86.74 ± 3.20

76.34 ± 5.60

90.96 ± 3.43

80.73 ± 3.33*

91.27 ± 1.51

72.65 ± 7.34*

Mean Burst Spike Rate (spikes/s)

34.75 ± 4.43

44.85 ± 8.02

70.56 ± 22.46

42.67 ± 9.09

41.08 ± 7.12

27.84 ± 10.20

51.13 ± 7.56

33.38 ± 12.41

49.12 ± 10.56

53.08 ± 36.94

Burst Interspike Interval (ISI) (ms)

64.1 ± 5.44

76.66 ± 10.7

91.99 ± 19.16

96.57 ± 15.53

72.20 ± 11.62

132.11 ± 23.90*

63.42 ± 13.40

140.63 ± 27.85**

64.22 ± 10.16

189.79 ± 26.99***

Mean Spike Rate (spikes/s)

0.95 ± 0.27

1.19 ± 0.34

0.91 ± 0.35

0.72 ± .16

0.91 ± 0.31

0.69 ± 0.14

0.86 ± 0.20

0.78 ± 0.19

0.67 ± 0.04

0.58 ± 0.08

Burst Duration (s)

2.01 ± 0.18

1.93 ± 0.27

2.20 ± 0.27

1.82 ± 0.16

1.94 ± 0.23

2.25 ± 0.19

2.08 ± 0.14

2.10 ± 0.35

2.19 ± 0.41

2.30 ± 0.39

P2 Saline

P2 ITOX

P4 Saline

P4 ITOX

P6 Saline

P6 ITOX

P8 Saline

P8 ITOX

P10 Saline

P10 ITOX

Wave Bursts

Burst Duration

1.96 ± 0.11

1.91 ± 0.22

2.07 ± 0.42

1.85 ± 0.16

1.93 ± 0.23

2.14 ± 0.20

2.14 ± 0.18

2.10 ± 0.23

2.30 ± 0.52

2.52 ± 0.24

Burst Spike Rate

32.47 ± 6.22

40.18 ± 7.08

56.85 ± 18.58

36.04 ± 8.14

37.14 ± 5.34

21.63 ± 2.60*

46.52 ± 5.34

25.38 ± 7.12*

51.40 ± 12.62

40.06 ± 25.54

Burst ISI

62.59 ± 5.72

70.74 ± 8.87

70.78 ± 16.25

90.59 ± 12.28

67.71 ± 9.09

131.06 ± 21.76**

58.05 ± 9.48

132.27 ± 23.50**

52.65 ± 3.44

176.17 ± 20.79***

Burst Spike Rate from ISI

16.26 ± 1.37

14.61 ± 1.69

15.02 ± 2.90

12.01 ± 1.78

15.98 ± 2.24

8.11 ± 1.33**

18.38 ± 2.24

8.13 ± 1.46***

19.03 ± 1.24

5.79 ± 0.72***

Interburst Interval (IBI)

144.15 ± 18.28

126.84 ± 17.00

155.55 ± 35.60

167.87 ± 27.30

154.43 ± 27.59

143.91 ± 10.34

164.20 ± 10.60

113.82 ± 36.40

184.21 ± 6.98

161.42 ± 49.09

Maximum Burst Spike Rate

489.97 ± 73.78

480.11 ± 127.77

674.90 ± 174.07

430.41 ± 162.99

529.49 ± 66.71

456.35 ± 140.18

504.49 ± 98.33

395.51 ± 189.97

634.39 ± 62.69

563.02 ± 291.44

% Neurons Bursting Per Second In Waves

4.01 ± 0.41

4.52 ± 0.47

4.84 ± 0.30

4.14 ± 0.46

5.01 ± 0.35

3.04 ± 0.15***

5.24 ± 0.42

3.71 ± 0.48*

8.80 ± 0.93

3.48 ± 0.26***

Bursts Per Neuron Per Wave

1.04 ± 0.02

1.05 ± 0.03

1.10 ± 0.07

1.06 ± 0.06

1.12 ± 0.07

1.17 ± 0.14

1.21 ± 0.10

1.04 ± 0.02

1.45 ± 0.25

1.04 ± 0.01

Percent Burst Time >10 Hz

59.71 ± 3.32

57.97 ± 4.15

59.14 ± 5.44

53.51 ± 3.54

60.17 ± 3.60

42.37 ± 3.22***

66.71 ± 3.15

39.72 ± 4.91***

66.80 ± 0.50

33.53 ± 4.69***

Non-Wave Bursts

Burst Duration

2.05 ± 0.30

1.99 ± 0.34

2.23 ± 0.44

1.83 ± 0.18

1.99 ± 0.24

2.34 ± 0.20

1.97 ± 0.16

2.09 ± 0.46

1.94 ± 0.08

2.14 ± 0.49

Burst Spike Rate

34.96 ± 3.72

49.24 ± 10.32

115.04 ± 45.04

50.14 ± 13.36

46.14 ± 10.29

31.83 ± 17.60

59.78 ± 11.62

42.19 ± 21.54

28.96 ± 9.93

60.44 ± 40.62

Burst ISI

65.37 ± 10.46

77.49 ± 14.19

81.52 ± 29.22

103.91 ± 19.46

77.88 ± 14.38

144.92 ± 32.94*

70.38 ± 21.99

147.73 ± 33.29*

101.08 ± 55.77

207.30 ± 20.09

Burst Spike Rate from ISI

34.96 ± 3.72

49.24 ± 10.32

115.04 ± 45.04

50.14 ± 13.36

46.14 ± 29

31.83 ± 17.60

59.78 ± 11.62

42.19 ± 21.54

28.96 ± 9.93

60.44 ± 40.62

Maximum Burst Spike Rate

437.86 ± 100.60

479.49 ± 209.59

1016.96 ± 333.70

477.51 ± 143.01

496.95 ± 117.20

583.86 ± 188.27

607.25 ± 47.91

477.49 ± 194.47

624.04 ± 310.54

751.96 ± 354.12

Percent Burst Time >10 Hz

57.56 ± 4.76

56.82 ± 4.32

56.22 ± 4.62

52.17 ± 4.42

59.43 ± 4.75

40.89 ± 3.93**

65.61 ± 3.19

39.29 ± 4.67***

51.51 ± 12.81

32.16 ± 3.04

Wave size (% of channels)

29.01 ± 1.77

28.55 ± 1.69

29.26 ± 2.07

24.32 ± 0.98*

33.48 ± 2.03

13.33 ± 0.50***

41.11 ± 2.56

17.21 ± 0.57***

46.83 ± 6.44

18.28 ± 1.14***

Wave frequency (waves/min)

2.15 ± 0.20

2.23 ± 0.25

1.60 ± 0.12

2.92 ± 0.37*

2.26 ± 0.56

4.43 ± 0.90

1.29 ± 0.13

5.63 ± 1.35**

0.79 ± 0.19

3.19 ± 0.97