Figure 4
From: Neuropilins define distinct populations of neural crest cells
Nrp1 and Nrp2 are expressed in distinct populations of trunk NCCs. (A-E) Lateral view of whole mount immunofluorescence staining of Nrp1, Nrp2 and trunk NCCs at E9.5. (C) GFP labelled all NCCs migrating in the anterior half of the somite. (D) Nrp1 was expressed in many GFP-positive NCCs and intersomitic vessels. (E) Nrp2 was expressed in the anterior half of the somite and within NCCs. Notably, Nrp1 had high expression in NCCs that had either low or negative Nrp2 expression (white arrow). (F) Transverse sections through the anterior half of the somite over the forelimb region showed that neuropilins were expressed within different populations of NCCs. NCCs migrating through the somite in and around the anlage of the dorsal root ganglia (Fi-i′′′) consisted of cells with high Nrp2 and no Nrp1 expression (inset) and conversely, with high levels of Nrp1 and no expression of Nrp2 (white arrow). (Fii-ii′′′) NCCs migrating towards the dorsal aorta (inset) or condensing in the region of the sympathetic ganglia (white arrow) preferentially expressed Nrp1. av, anterior cardinal vein; bv, blood vessel; da, dorsal aorta. Scale bars = 100 μm.