Figure 3
From: Neuropilins define distinct populations of neural crest cells
Nrp1 and Nrp2 are expressed in trunk NCCs at the same axial level. (A-C) Lateral view of whole mount in situ hybridisation at E9.5 for Sox10, Nrp1, and Nrp2. (A) Sox10 whole mount in situ hybridisation specifically labelled NCCs migrating through the anterior half of the somite (black arrow). (B) Nrp1 in situ hybridisation labelled NCCs (black arrow), intersomitic blood vessels (white arrowhead) and the dorsal aorta (asterisks). (C) Nrp2 was expressed within the anterior half of the somite (black arrow). (D-I) Longitudinal sections of Nrp1 and Nrp2 in situ hybridisation over the hind limb counterstained with antibodies to p75. (D, G) p75 expression labelled Sox10-positive NCCs in the anterior half of the somite (white and black arrow, respectively). (E, H) Nrp1 had its highest expression in cells that also expressed p75 (black and white arrows), and was also expressed in the somitic tissue and intersomitic blood vessels. (F, I) Nrp2 was expressed in the anterior half of the somite within NCCs and also in the somitic tissue (black and white arrows). a, anterior; IS, in situ hybridisation; p, posterior. Scale bars = 100 μm.