Figure 10
From: Axon-Schwann cell interactions during peripheral nerve regeneration in zebrafish larvae
Role of ErbB signaling in axonal regeneration. Three days post fertilization larvae were neurectomized (circles indicate position of neurectomy) after incubation with 0.05% of DMSO (controls) or 5 μM of AG1478 from 60 hpf to 5 dpf (48 hpn); this treatment does not affect Schwann cell survival but blocks ErbB signaling during the regeneration phase. Nerve regeneration in control (A-C) and drug treated larvae (D-F) was evaluated after 10 hpn, 14 hpn, and 48 hpn. The insets in C and F show the defasciculation of the regenerated nerve in treated larvae in comparison to controls. (G-I) Quantitative analysis of axonal extension after 10 hpn (G), 14 hpn (H), and 48 hpn (I) in control and AG1478 treated larvae. *P <0.05; ***P <0.001. (J) In 30% of ErbB inhibitor treated larvae, the non-neurectomized contralateral nerve appears defasciculated in comparison to the control nerve. Scale bar: C, F: 200 μm; A, B, D, E, J; insets in C and F: 100 μm.