Figure 3
From: Pioneer midbrain longitudinal axons navigate using a balance of Netrin attraction and Slit repulsion
Medial longitudinal fasciculus axons shift away from the midline in deleted in colorectal cancer receptor mutants. (A-D) Deleted in colorectal cancer (DCC) mutant and control embryonic day 10.5 embryos labeled by whole-mount βIII-tubulin antibody (A,C) and diI labels (B,D). (E) Axon positions were quantified, normalized for variations in embryo size, and compared to the Netrin1 data from Figure 3. Medial longitudinal fasciculus axons shifted dorsally in DCC−/− mutants (n = 12), compared to controls (DCC+/+, n = 9; DCC+/−, n = 9), but significantly less than in Netrin1 mutants. Error bars show SEM; significance was measured using the t-test. *P < 0.05; **P < 0.01. Scale bar in (D) indicates 200 μm and applies to (A-D).