Figure 2
From: Transcriptional control of GABAergic neuronal subtype identity in the thalamus
Helt expression is sufficient for rostral thalamic (rTh) subtype specification. Ectopic expression of the rTh transcription factor Helt in the caudal thalamic (cTh) glutamatergic progenitor domain at E2.5, suppresses Neurog2 and Lhx9 expression, indicative of a loss of glutamatergic progenitor identity (A). cTh progenitors expressing ectopic Helt, acquire expression of all rTh markers tested: proliferative zone (Ascl1) and postmitotic compartment (Gata2, Tal1 and Sox14) (B). PanGABAergic markers are also induced (Gad1 and Reelin) (C). Ectopic Helt expression in the prethalamic (pTh) GABA progenitor domain fails to induce rTh subtype identity, suggesting that other co-factors may be required (D). Schematic drawing summarizing the effect of ectopic Helt expression in the three progenitor domains of the thalamus: cTh, rTh and pTh (E). Scale bars: 100 μm.