Figure 7
From: Temporal and spatial requirements of Smoothened in ventral midbrain neuronal development
Persistent loss of neurons in the red nucleus, oculomotor nucleus and the serotonergic neurons, but not dopaminergic neurons, in Shh-Cre;Smofl/flmutants at embryonic day (E)12.5 to post-natal day (P)0. (A-B’) Nurr1 and tyrosine hydroxylase (TH) staining from E12.5 and P0 Shh-Cre;Smofl/fl mutants showed no change compared with controls. (C-D’) Brn3a, (E-F’) Islet1 and (G-H’) 5-Hydroxytryptamine (5-HT) revealed selective reduction of neurons in the red nucleus, neurons in the oculomotor nucleus, and serotonergic neurons in the raphe nuclei at E12.5 and P0 after removal of Smoothened. (F-F’) Dashed line indicates the midline. Scale bars: (G’) 50 μm, applied to A-A’, C-C’, E-E’ and G-G’). (I-J) Quantification of TH+ and Nurr1+ cells at E10.5, E12.5 and P0 confirmed the transient reduction of committed DA progenitors and DA neurons in Shh-Cre;Smofl/fl mutants. (K-M) Quantification of the reduction of neurons in the red nucleus, oculomotor nucleus, and the raphe nuclei (serotonergic neurons) at E12.5 and P0 in Shh-Cre;Smofl/fl mutants. Student’s t test, n = 3 or 4.