Figure 5
From: Temporal and spatial requirements of Smoothened in ventral midbrain neuronal development
Transient reduction of dopaminergic (DA) progenitors in Shh-Cre;Smofl/flmutants. (A-B’) At embryonic day (E)9.5, Shh-Cre;Smofl/fl mutants showed no detectable reduction in progenitors expressing (A’) Lmx1a/Nkx2.2, or (B’) Foxa2/Nkx6.1. (C-F’) By contrast, Shh-Cre;Smofl/fl mutants showed a significant reduction in (C-C’, D-D’) Foxa2+, (C-C’) Nkx2.2+, (D-D’) Nkx6.1+, and (F-F’) Nurr1+ progenitors at E10.5, whereas (E-E’) Sox2+ progenitors were unchanged. (E-F’) Note that the DA neurons were also reduced at E10.5. (G’,H’) Foxa2+ and (H’) Nkx6.1+ progenitors from E12.5 Shh-Cre;Smofl/fl mutants showed no change compared with (G,H) controls, whereas (G’) the reduction in Nkx2.2+ progenitors persisted at E12.5. Scale bars: (A) 50 μm (applied to A to H’). (I-L) Quantification of total number of (I) Lmx1a+, (J) Foxa2+, (K) Nkx2.2+ and (L) Nkx6.1+ progenitors confirmed their transient reduction at E10.5. Student’s t test, n = 3 or 4. (M-N) Quantification of number of cells in each progenitor domains at E10.5 showed a consistent reduction from D1 to D4 domains at E10.5.