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Figure 4 | Neural Development

Figure 4

From: Histamine up-regulates fibroblast growth factor receptor 1 and increases FOXP2 neurons in cultured neural precursors by histamine type 1 receptor activation: conceivable role of histamine in neurogenesis during cortical development in vivo

Figure 4

HA up-regulates FGFRs transcripts. RT-PCR analysis of the effect of 100 μM HA on FGFRs expression. Kinetics of the effect of HA on FGFR1 (A), FGFR2-IIIC (D) and FGFR3 (G) expression, normalized by GAPDH; the increases are presented as percentage of the expression obtained in control conditions for each time point. **P <0.01 and ***P <0.001 versus control. (B) Pharmacological analysis of HA effect on FGFR1 expression at 30 minutes. The H1R antagonist chlorpheniramine (Chlorph.) prevented the increase in FGFR1 expression caused by HA, whereas cimetidine (Cimet.) was uneffective. (E) FGFR2-IIIC expression significantly increased after 120 minutes of HA treatment; both chlorpheniramine and cimetidine were able to block such increase. (H) HA increased FGFR3 transcripts, but such elevation was not sensitive to either chlorpheniramine or cimetidine. **P <0.01 versus control; aP <0.01 versus its corresponding condition in the absence of HA. (C, F and I) Representative bands for the conditions shown in B, E and H, respectively, including a positive control (adult cerebral cortex) and PCR in the absence of retrotranscription (-RT). FGFRs, fibroblast growth factor receptors; HA, histamine.

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Neural Development

ISSN: 1749-8104