Figure 2

Reducing XMAP215 function leads to an increase in microtubule (MT) plus-end velocity in growth cones. (A-F) Data and quantitative analysis of the mean values per growth cone of EB1-GFP comets in control and XMAP215 knockdown (KD) (see Additional file 2). (G, H) Quantification of MT growth track parameters after adding XMAP215 mRNA to KD. (I-J) Quantification of MT plus-end growth velocities in neural crest and axons. Box-and-whisker plots indicate the mean (diamond), median, extrema and quartiles. *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant. N = growth cone/cell number. Bar is 5 μm.