Figure 1
From: Sympathetic neurons and chromaffin cells share a common progenitor in the neural crest in vivo
Single-cell EP of GFP-DNA into the dorsal neural tube. (A) Diagram showing the two-step procedure for single-cell EP, beginning by plasmid microinjection and followed by application of ventro-dorsally directed electric pulses. Note position of electrodes, injection site, and position of single electroporated cell. (B,C) Live-visualization in ovo at 3.5 h (B) and 24 h (C) after EP. (D) Histological cross section showing confocal analysis of a single GFP-expressing cell in the dorsal NT 3.5 h after EP visualized with an anti-GFP-antibody, combined with DAPI nuclear staining (blue). D, D’, D”, and D”’ show four representative focal planes out of 23 planes from a Z-stack of a 10 μm thick section. Samples were optically screened at 0.35 μm increments. D”” shows orthogonal projections. (E) Summarizes results of 69 experiments, with 71% successful single-cell-EPs verified by immunohistology, and 27.5% of the cases, where two cells were seen. In 1.5% of the cases, GFP was visualized in three cells. Scale bar: 10 μm. NT, neural tube; S, somites.