Figure 3

Retinogeniculate projections in rostral and caudal sections of vldlr^−/−; lrp8^−/−dLGN. Retinogeniculate projections in a P16 vldlr^−/−;lrp8^−/− mutant were labeled by intraocular injection of fluorescently conjugated CTB. The left eye was injected with Alexa Fluor 594 CTB and the right eye was injected with Alexa Fluor 488 CTB. Several sections of the same LGN from rostral to caudal are shown. Projections originating from the contralateral retina are labeled in red and projections originating from the ipsilateral retina are labeled in green. Arrowheads indicate regions of binocular, abnormal retinal projections. In central and caudal LGN sections, this region of ectopic innervation is in the dorsomedial pole of mutant dLGN. However, in extreme caudal sections, the ectopic innervation invades more lateral dLGN regions. Scale bar = 200 μm. CTB, cholera toxin B subunit; dLGN, dorsal LGN; LGN, lateral geniculate nucleus.