Figure 5

unc-36 regulates presynaptic morphology . (A, B) unc-36 mutants also had slightly enlarged puncta, and also suppressed the defects in nid-1. (C) unc-2;unc-36 mutants appeared similar to the single mutants, but were not further enlarged. (D) Suppression of nid-1 was not enhanced by simultaneous loss of both unc-2 and unc-36. (E) A deletion in the ptp-3A specific coding region, tm352, causes a nid-1 like defect in synapses, which is also suppressed by loss of unc-36 (F). (G) Broadly replacing UNC-36 function via transgene efficiently rescued the enlarged puncta found in unc-36(e251) mutants. (H) Specific expression of UNC-36 solely in the D-type motor neurons was also sufficient to reduce the puncta enlargement found in unc-36(e251) . (I, J) Treatment of wild type animals (I) or nid-1(cg119) (J) with the α2/δ-subunit antagonist, gabapentin, resulted in enlarged puncta similar to those found in e251. (K) The puncta area by genotype are plotted as the mean (circle) with 5 to 95% confidence interval (whiskers). All animals in these micrographs are young adults (1 to 2 days post 4th larval stage (L4)).