Figure 7

Analysis of ephrin A5 expression in two ROIs in the neocortex of control and enucleated mice. (A) Medial parietal cortex regions of interest overlaid on a representative coronal brain illustration. (B) Percent area fraction of cells expressing ephrin A5 within ROI1, located within the medial parietal cortex. A sharply increased percentage of transcript is observed in enucleated cases (31.46 ± 2.2%; df = 12; n = 7) when compared to control animals (0.49 ± 0.18%; df = 12; n = 7; P < 0.001; two-sample t-test). (C) Percent area fraction of cells expressing ephrin A5 within ROI2, located immediately lateral to ROI1. Percentage of ephrin A5 transcripts within the putative somatosensory cortex of enucleated animals (61.77 ± 1.18%; df = 12; n = 7) are not significantly different from controls (61.29 ± 1.00%; df = 12; n = 7; P = 0.47). CC: corpus callosum; CP: caudoputamen; CTX: cortex; df: degrees of freedom; LSr: lateral septal nucleus; ROI: region of interest.