Conditional over-expression of Mdka. (A) Induced fluorescence in a Tg(pHSP70/4:mdka: egfp) larva at 72 hpf after heat shock treatment at 24 hpf. (B) Western blot stained with antibodies against EGFP showing induction of the fusion protein at 72 hpf after heat shock at 24 hpf. Tg, heat shock-treated, transgenic siblings; WT, heat shock-treated, wild-type embryos. (C) Mdka gain of function results in marked retinal overgrowth at 30 hpf, which is largely resolved by 72 hpf (D). Tg, heat shock-treated embryos from the Tg(pHSP70/4:mdka:egfp) line; WT, heat shock-treated wild-type siblings. Scale bar equals 50 μm.