Figure 2

Transfection is limited to auditory nuclei as seen by EGFP fluorescence. (A) Dissected chick brainstem at E10 following in ovo electroporation at E2. Bilateral auditory nuclei are EGFP positive, as are the axons connecting NM to contralateral NL (white arrowheads). (B) Coronal section of E10 chick brainstem (left side) at approximately the level shown by a black dashed line in (A) shows transfection is extensive in but not limited to NM cells and their axons. In the section shown here, three NL cells and their bipolar tufts of dendrites within the cellular monolayer are visible by EGFP fluorescence. (A, B) White arrowheads delineate the margins of contralateral NM axons, and are oriented in (B) to indicate anterograde direction from NM origin to contralateral target. Scale bar = 1 mm in (A) and 100 μm in (B).