Figure 5

Aberrant patterns of cell proliferation in Neurog1/2^−/−mutants. (A) to (H) BrdU-labeled S-phase progenitors at embryonic day (E) 11.5 (A) to (D), and E13.5 (E) to (H) in wild-type (A,E), Neurog1^−/− (B,F), Neurog2 ^KI/KI (C,G), and Neurog1/2^−/− (D,H) OBs. (I) to (K) Illustration schematizing areas where counts of BrdU-labeled S-phase progenitors were performed (I). Quantitation of the ratio of BrdU⁺ cells in the OB compared with the dorsal telencephalon at E11.5 (J) and E13.5 (K). Asterisks denote P <0.05. (L) to (N) Illustration schematizing areas where counts of BrdU-labeled S-phase progenitors were performed (L). Quantitation of the ratio of BrdU⁺ cells in the ventral compared with the dorsal telencephalon at E11.5 (M) and E13.5 (N). (O) to (V) Co-labeling of BrdU⁺ S-phase progenitors (green) with either Tbr2 (red, O to R) or Dlx2 (red, S to V) in E13.5 wild-type (O,S), Neurog1^−/− (P,T), Neurog2^KI/KI (Q,U), and Neurog1/2^−/− (R,V) embryos. dTel, dorsal telencephalon; OB, olfactory bulb; pOB, presumptive olfactory bulb; pOBLS, presumptive olfactory bulb like structure; vTel, ventral telencephalon. Scale bars: 250 μm (A) to (H), (O) to (V).