Figure 2From: Rapid genetic targeting of pial surface neural progenitors and immature neurons by neonatal electroporationPerinatal superficial tectum electroporation. (A) An en face preparation is shown of the perinatal mouse brain. The left optic tectum (that is, superior colliculus) is outlined in white. (B) A representative image of a day 2 postnatal mouse pup is shown prior to injection into the superior colliculus. (C) A day 2 postnatal mouse pup injected with plasmid DNA and fast green dye is shown, highlighting unilateral labeling of the tectal surface. Images depicting (D) parallel or (E) forward-directed electrode orientation are shown. (F) A maximum intensity projection of the superior colliculus is shown depicting EGFP (green) and nuclear TagBFP (actually blue, but pseudocolored red) as imaged with a macroconfocal microscope. (G) Quantification of electroporated cells (n = 3 mouse pups each) is shown. (H) Electroporated EGFP labels a cohort of cell types at the pial surface. Scale bar measures 100 μm in H.Back to article page