|
treatment.
|
Average number of cells per DRG at five dpf
|
|---|
| |
BrdU incorporation at two dpf
|
BrdU incorporation at five dpf
|
|---|
| |
Elavl+
|
BrdU+
|
Elavl+/BrdU+
|
Elavl+
|
BrdU+
|
Elavl+/BrdU+
|
|---|
|
DMSO
|
3.26 ± 0.27
|
2.11 ± 0.47
|
0.09 ± 0.05 (4/144)
|
3.74 ± 0.20
|
1.98 ± 0.33
|
0.04 ± 0.02 (2/204)
|
|
DAPT
|
3.86 ± 0.17a
|
2.67 ± 0.47
|
0.19 ± 0.05 (9/187)
|
4.32 ± 0.15b
|
1.69 ± 0.41
|
0.04 ± 0.04 (2/226)
|
- Animals were incubated with BrdU for 1.5 hours at two dpf or five dpf. Both sets of larvae were incubated in DMSO or DAPT from two to five dpf. Animals were euthanized and fixed at five dpf for analysis. There was a significant increase in the total number of Elavl + neurons after DAPT treatment, but no change in proliferation. Numbers represent mean cell counts from nine to ten larvae per condition and four to six ganglia per larvae, ±SEM, aP = 0.05, bP = 0.03, Student’s t test. The number of BrdU+/Elav + compared to total Elav + cells is also shown in parentheses. BrdU, bromodeoxyuridine; DAPT, N-`N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester; DMSO, dimethyl sulfoxide; dpf, days postfertilization.
