Postembryonic neuronal addition in Zebrafish dorsal root ganglia is regulated by Notch signaling

Table 1 Proliferation of cells associated with the dorsal root ganglia (DRG)

treatment.	Average number of cells per DRG at five dpf
	BrdU incorporation at two dpf			BrdU incorporation at five dpf
	Elavl+	BrdU+	Elavl+/BrdU+	Elavl+	BrdU+	Elavl+/BrdU+
DMSO	3.26 ± 0.27	2.11 ± 0.47	0.09 ± 0.05 (4/144)	3.74 ± 0.20	1.98 ± 0.33	0.04 ± 0.02 (2/204)
DAPT	3.86 ± 0.17^a	2.67 ± 0.47	0.19 ± 0.05 (9/187)	4.32 ± 0.15^b	1.69 ± 0.41	0.04 ± 0.04 (2/226)

Animals were incubated with BrdU for 1.5 hours at two dpf or five dpf. Both sets of larvae were incubated in DMSO or DAPT from two to five dpf. Animals were euthanized and fixed at five dpf for analysis. There was a significant increase in the total number of Elavl + neurons after DAPT treatment, but no change in proliferation. Numbers represent mean cell counts from nine to ten larvae per condition and four to six ganglia per larvae, ±SEM, ^aP = 0.05, ^bP = 0.03, Student’s t test. The number of BrdU+/Elav + compared to total Elav + cells is also shown in parentheses. BrdU, bromodeoxyuridine; DAPT, N-`N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester; DMSO, dimethyl sulfoxide; dpf, days postfertilization.

ISSN: 1749-8104