Figure 4

Loss-of-function by morpholino antisense oligonucleotides. (A-B) Setting and electrodes utilized for electroporation at ED1.5. (C-D) Setting and electrodes utilized for electroporation at ED3-4. (E-G) Images from embryos electroporated with fluorescein-labeled morpholinos at ED1.5. (E) Dorsal view of a whole mounted embryo fixed immediately after electroporation; (F) Side view of a whole-mounted embryo fixed 24 h after electroporation; (G) transversal section of the eye of an embryo fixed 24 h after electroporation. Arrows indicate morpholino incorporation (green) into retinal progenitor cells. (H) Flat mount of a retina electroporated with morpholino at ED4 and collected 24 h later. Abbreviations: cf: choroid fissure; onh: optic nerve head.