Figure 4
From: Normal sulfation levels regulate spinal cord neural precursor cell proliferation and differentiation
Sodium chlorate induces G2 arrest of spinal cord neural precursor cells. (A-F) Neurospheres grown as high density culture in the presence of EGF and FGF2 for one week were dissociated, and single cells were plated for 2 h on a poly-DL-ornithine substrate for further analysis. The percentage of Nestin-positive cells was not altered upon NaClO3 addition (n = 6). In contrast, the relative number of βIII-Tubulin-positive neurons was significantly enhanced (n = 5; P <0.05). NaClO3 did not affect the proportion of Caspase3-positive cells (n = 4). (G, I) BrdU incorporation analysis was performed after one day and after seven days. NaClO3 did not affect the BrdU incorporation rate for either time point investigated (1div: n = 4; 7div: n = 5). (H, J) Moreover, the relative number of pH3-positive cells was also not changed in response to NaClO3. Strikingly, the percentage of cells in the G2 and M-phase of the cell cycle was increased and decreased, respectively (n = 4; P = 0.0002). Hoechst 33528 was added to visualize the cell nuclei. Scale bar: 25 μm (H-H”), 50 μm (A, B, C, G).