Figure 3
From: Normal sulfation levels regulate spinal cord neural precursor cell proliferation and differentiation
Sodium chlorate affects clonal neurosphere formation from primary spinal cord cells. (A, B) Neurospheres were grown under clonal conditions in the presence of EGF and FGF2. The addition of NaClO3 resulted in smaller neurospheres in comparison to the solvent control. (C) Moreover, the quantification of neurospheres revealed a significantly reduced number of neurospheres in the presence of NaClO3 (n = 5; P <0.05). (D) Consistent with this observation, the total cell number in high density cultures was strongly reduced upon NaClO3 treatment after one week (n = 4; P <0.01). (E) Analysis of EGF- and FGF2-dependent neurosphere formation showed that, under both growth factor conditions, the addition of NaClO3 resulted in a significantly smaller number of neurospheres (n = 3; P <0.05 for both conditions). (F) Using neurosphere size, based on the neurosphere diameter, in the presence or absence of NaClO3, the neurospheres were sorted into different categories. The size distribution histogram shows that NaClO3 treatment led to a higher number of small neurospheres. However, some large neurospheres were still detectable. Scale bar: 100 μm.