Figure 2
From: Normal sulfation levels regulate spinal cord neural precursor cell proliferation and differentiation
Sodium chlorate efficiently reduces the expression level of the sulfation-dependent 473HD-epitope. (A) Western blot analysis of neurosphere detergent extracts, showing a reduced 473HD expression level upon NaClO3 treatment. The expression level of different RPTPβ/ζ isoforms, known 473HD carrier proteins, did not appear to be changed in the presence of NaClO3. (B) Immunohistochemical detection of the 473HD-epitope and RPTPβ/ζ isoforms on neurosphere cryosections confirmed the reduced 473HD expression levels in NaClO3-treated cultures. Here, the immunoreactivity for the 473HD carrier proteins seemed to be enhanced. Hoechst 33528 was used to visualize the cell nuclei. Scale bar: 50 μm.