Postnatal laminar distribution of cortical interneuron subtypes. (A-F) Immunohistochemical detection of interneuron subtypes carried out for calbindin (A, D), calretinin (B, E), and parvalbumin (C, F) positive cells in the adult cortex. (G) The analysis was carried out on a cortical segment of 400 μm length as shown. Cortical sections were divided between the marginal zone and white matter (WM) in four horizontal bins (B1 to B4) and immunolabeled cells of each bin counted. Scale bar: 100 μm. (H) Quantification and laminar comparison between WT and Foxc1hith/hith mutants. Error bars indicate standard error. No significant differences in the laminar distribution of interneuron subtypes were observed and the P-values for each pairwise comparison is indicated below the bar diagrams.