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Figure 3 | Neural Development

Figure 3

From: Analysis of cell identity, morphology, apoptosis and mitotic activity in a primary neural cell culture system in Drosophila

Figure 3

Proliferation and cell division markers in third instar larval brains and primary cell cultures. (A, B) The pcna-GFP line positively-labels cells in S-phase (green) co-labeled with anti-Elav (red) and DAPI. (C, D) Immunostaining with anti-phosphorylated Histone3 antibody (PH3, green) shows mitotic cells in larval brains (C) and in culture (D). (E, F) BrdU (green) pulse for 3.5 h in third instar larval brains (E) and 4-h-pulse in primary culture mark proliferating cells, that have replicated DNA (F; arrows).

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Neural Development

ISSN: 1749-8104