Panx1 regulates primary NSC/NPC proliferation. (A) Outline of neurosphere treatments in vitro. Growth factors (hEGF and bFGF) were added every two DIV beginning at DIV0, while the Panx1 blocker probenecid was added at DIV1 to a concentration of 1 mM. Sphere diameter was measured at DIV7. (B) Brightfield images of vehicle and probenecid treated spheres (i). A significant reduction in neurosphere diameter was observed following probenecid treatment compared to vehicle treatment (41.85 ± 1.649 μm and 93.97 ± 5.089 μm respectively, P < 0.0001 for non-parametric t test, N = 12) (ii). DIV, days in vitro; NPC, neural progenitor cells; NSC, neural stem cells; Panx1, pannexin 1.