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Figure 5 | Neural Development

Figure 5

From: Neurogenesis in the central olfactory pathway of adult decapod crustaceans: development of the neurogenic niche in the brains of procambarid crayfish

Figure 5

The emergence of the niche core during hatching, immunolocalization of tyrosinated tubulin and the nuclear stain YOYO (magenta). (A) Ventral view of the brain showing two forming protoniches that are located bilaterally symmetrical on the surface of the deutocerebrum (square on the right, circle on the left) close to the medial giant neuron (GN). In the brain, clusters composed of neuroblasts and their progeny are still patchily dispersed (white stars). Arrowheads identify nerve roots. (B) Higher magnification of the protoniche boxed in (A). The arrow points to microtubules that converge in the center of the protoniche. (C) Another example of a protoniche located close to the giant neuron (GN). (D) Deeper focus of the specimen in (C) showing a cell in M-phase (arrow). (E) Within the protoniche, cells that surround the central fibrous area have very condensed, small nuclei (arrows). The cells in the cap are spherical but vary greatly in size. (F) Dividing cells in the protoniche close to surrounding the central fibrous area (arrows). Scale bars: 20 μm (A, B, E), 10 μm (C, D, F).

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