Effects of Ferret VAChT-SAP treatment on starburst amacrine and retinal ganglion cell ablation. (A,B) Treatment with Ferret VAChT-SAP leads to ablation of ChAT(+) starburst amacrine cells (SACs) and loss of vesicular acetylcholine transporter (VAChT) expression. (C-F) Brn3a(+) and NeuN(+) retinal ganglion cells (RGCs) are also ablated, although to a lesser degree than SACs, by Ferret VAChT-SAP treatment ((C,E) photomicrographs; (D,F) quantification). Data reflect means ± standard errors of the mean. Statistics reflect two-tailed P-values calculated from independent two sample t-tests. 'Merge' panels are counterstained with DAPI to reveal cytoarchitecture. GCL, ganglion cell layer; IPL, inner plexiform layer, INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; PRL, photoreceptor layer. Scale bars: 100 μm.