NKCC1 affects migration of neuroblasts in a GABA
-independent manner. (A) Plot of the average migration speed of neuroblasts from the control, bumetanide and shNKCC1 groups (hatched bars) and these groups after incubation in 10 μM gabazine (+ Gz; solid bars). Error bars indicate standard error of the mean. (B) Normalized cumulative plot for the averaged migration speed in the groups displayed in (A). The shift to the leftward direction in the bumetanide and shNKCC1-transduced cells treated with gabazine compared to the gabazine treated control group indicates GABAA-receptor-independent effects (solid lines). (C) Analysis of the directionality of the migration during the experimental session in control, bumetanide-treated and shNKCC1-transduced cells incubated in gabazine. Analysis of directionality of migration as in Figure 3C.