Figure 2
From: NKCC1 controls GABAergic signaling and neuroblast migration in the postnatal forebrain
Immunohistochemical staining for NKCC1 in organotypic cultured slices at 1 and 6 dpi. (A) Upper panel: photomicrograph showing the immunolabeling for NKCC1 (red) and GFP (green) in RMS neuroblasts transduced with the control non-target sequence after 1 dpi (left) and 6 dpi (right). Each immunolabeling image is accompanied by an image corresponding to its analysis of the product of the differences from the mean (PDM), which shows the correlated areas in yellow and the segregated ones in blue. Lower panel: photomicrograph showing the immunolabeling for NKCC1 (red) and GFP+ (green) in RMS neuroblasts transduced with the shNKCC1 sequence after 1 dpi (left) and 6 dpi (right), and their corresponding PDM analysis. Scale bars: 10 μm. (B) Normalized average (± standard error of the mean) number of neuroblasts showing correlated NKCC1 and GFP signals in cells transduced with the control and shNKCC1 sequences after 1 and 6 dpi. **P ≤ 0.01.