Punctate expression is observed in the cell bodies in the ganglion cell layer (GCL) and in the inner plexiform layer (IPL) of Thy1-YFP-NL2 retinas. (A) En face (top) view of a P21 Thy1-YFP-NL2 retina at the level of the RGC layer or at the IPL. (B) High magnification view of YFP-NL2 puncta in the GCL and IPL. Arrows indicate puncta on a RGC soma. (A, B) Maximal intensity projection encompassing 4.5 μm thickness. (C) Vertical slices through Thy1-YFP-NL2 retinas at various ages, showing punctate staining in the IPL. Faint expression in horizontal cells (HCs) was observed transiently during development. INL, inner nuclear layer. Maximal intensity projections of optical planes encompassing 5 to 8 μm thickness.