Figure 6

Bazooka is an intrinsic requirement for axon pathway choice. (A) Neuroblasts and newborn progeny in period-GAL4,UASGFP third instar larva brains labeled with anti-Bazooka (magenta). Newborn neurons were negative for green fluorescent protein (GFP)(arrow), indicating that period- GAL4 was not active in the ganglion mother cells (GMCs). (B) Mosaic analysis with a repressible cell marker (MARCM) bazooka loss-of-function (LOF) clone of the BAla1 lineage (green) re-expressing Bazooka protein (magenta) in mutant neurons (see inset). Note that the axon only entered the middle antennal cerebral tract (mACT) (arrow). (C) The percentage of axons entering the inner (i)ACT compared between control clones (0%, n = 28), bazooka LOF clones (91%, n = 11), and bazooka LOF clones re-expressing Bazooka protein only in vPNs (29%, n = 7). Asterisk indicates a significant decrease in the percentage of BAla1 LOF clones re-expressing Bazooka entering the iACT compared with the percentage of BAla1 LOF clones without Bazooka entering the iACT (P < 0.05). Scale bars: 15 μm