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Figure 5 | Neural Development

Figure 5

From: Targeted electroporation of defined lateral ventricular walls: a novel and rapid method to study fate specification during postnatal forebrain neurogenesis

Figure 5

Radial glial cells located in defined walls of the lateral ventricle specify distinct periglomerular neuron subtypes. (A-C) Representative immunostainings for the three neuronal subtype markers CR (A), TH (B), and CB (C). For all markers, a low magnification overview with DAPI as a nuclear counterstain is shown (left panel), as well as confocal pictures showing higher magnifications of GFP+ neurons positive for the three markers (right panels). (D-F) Quantification of the percentage of newborn neurons (that is, GFP+) expressing CR (D), TH (E) or CB (F), which derived from the lateral (red), dorsal (gray) or septal (blue) LV walls. Error bars represent standard error of the mean. *P < 0.05; **P < 0.01; ***P < 0.001; determined by unpaired t-test. Scale bars: 50 μm, 30 μm and 10 μm in the left, middle and right panels, respectively. CB, calbindin; CR, calretinin; EPO, electroporation; TH, tyrosin hydroxylase.

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Neural Development

ISSN: 1749-8104