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Figure 4 | Neural Development

Figure 4

From: RBP-J is not required for granule neuron progenitor development and medulloblastoma initiated by Hedgehog pathway activation in the external germinal layer

Figure 4

RBP-J deletion in granule neuron progenitors does not influence the formation of Hedgehog-pathway-dependent medulloblastoma. (A-L) Cerebellar tumours of Ptc1lox/lox;RBP-Jlox/lox;Math1-Cre mice (B,D,F,H,J,L) closely resemble those of Ptc1lox/lox;Math1-Cre mice (A,C,E,G,I,K). Comparison was based on overall morphology by haematoxylin and eosin staining (A,B), granule cell identity by immunofluorescence staining for Pax6 (C,D), proliferation by immunofluorescence staining for proliferating cell nuclear antigen (PCNA) (E,F) and Hh pathway activity by in situ hybridisation for Gli1 (G,H). The canonical Notch target Hes1 is expressed in tumours of both genotypes as assayed by immunofluorescence staining as it can also be upregulated in response to Hh signals (I,J). Hes5, however, which even when upregulated as a response to Hh signalling, requires RBP-J, is lost in most cells of Ptc1lox/lox;RBP-Jlox/lox;Math1-Cre tumours (K) while being strongly expressed in all cells of Ptc1lox/lox;Math1-Cre tumours (L). Both images in each pair were taken at the same magnification; scale bars represent 200 μm for haematoxylin and eosin, Gli1 and Hes5, and 50 μm for Pax6, Hes1 and PCNA staining.

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Neural Development

ISSN: 1749-8104