Figure 6

Localization of the NMDA receptor subunit dNR1 in the mushroom body of young adult Drosophila. Double stainings with anti-dNR1 (magenta) and anti-GFP (green) in brain sections of c739-GAL4, UAS-mCD8::GFP flies. (A, B) In the MBs of recently eclosed flies, dNR1 is detected in processes of the pedunculus and lobes corresponding to the α/β and γ cells, whereas the core region is not labeled. (C) In the MBs of 10-day-old flies, some KC bodies are positive for dNR1 (K) but the calyx (ca) shows a low level of immunoreactivity. In contrast, a high level of dNR1 is present in the lateral horn (LH) area where the projection neurons from the antennal lobes end. (D, E) In 10-day-old flies, anti-NR1 staining is scattered within the α/β and γ areas of the pedunculus (D) and with high intensity in the spur (sp) region of the MB (E). Scale bars: 20 μm.