Figure 7
From: Ascl1 is a required downstream effector of Gsx gene function in the embryonic mouse telencephalon
Olfactory bulb interneuron subtype specification in Gsx2 , Ascl1 and Gsx2;Ascl1 mutants. (A-H) Calretinin (CR) staining in the dLGE (A-D) and olfactory bulb (E-H) in E18.5 wildtype (A, E), Gsx2 mutant (B, F), Ascl1 mutant (C, G) and Gsx2;Ascl1 mutants (D, H). Note that CR cells in the dLGE and olfactory bulb are severely depleted in Gsx2 and Gsx2;Ascl1 mutants while there appear to be similar if not more numbers of CR neurons in the Ascl1 mutants compared to wild type. (J, L) GAD67 (glutamic acid decarboxylase (67 kDa)) staining is severely reduced in Gsx2 mutants (J) and nearly absent in Gsx2;Ascl1 mutants (L). (I, K) In comparison, Ascl1 mutants (K) show a more modest reduction in GAD67 staining but it is still quite severe when compared to the wild-type olfactory bulb (I). GCL, granule cell layer; GL, glomerular layer.