Figure 5
From: Role of the Wnt receptor Frizzled-1 in presynaptic differentiation and function
Wnt-3a induces presynaptic differentiation in cultured hippocampal neurons mediated by the Fz1 receptor. (A) Neurons at 9 DIV exposed to Wnt-3a for 1 h show elevated numbers of Bassoon clusters detected by immunofluorescence. Addition of the soluble Fz1-CRD prevents this induction. (B) Synaptic vesicle recycling is visualized by FM4-64 uptake (11 DIV). One-hour treatment with Wnt-3a induced a significant increase in the number of FM4-64-positive sites, which was prevented by co-treatment with the Fz1-CRD. (C) Wnt-3a induces the number of VGlut1 clusters in neurons at 14 DIV, which was prevented by co-treatment with Fz1-CRD. Error bars indicate standard error of the mean. * P < 0.05; ** P < 0.01.